Cy3-UTP 抑制剂 BMPO Leukotriene D4 荧光染料 DMAPP 定制合成
Cy3-UTP可以取代UTP作为T7 RNA聚合酶的底物,通过体外转录产生标记的探针。在Cy3-UTPs的参与下,生成的组装mRNA将发出橙色的荧光。此外,Cy3标记的mRNA在紫外光下很容易看到,无需在凝胶电泳后进一步染色。由这些方法产生的探针适合于多色荧光分析,特别是与Cy3-UTP(Cyanine 3-UTP)结合的双色表达阵列。
Cy3-UTP(Cyanine 3UTP) can replace UTP as a substrate for T7 RNA polymerase and generate labeled probes through in vitro transcription. The resulting assembled mRNA will emit orange fluorescence with the involvement of Cy3-UTP. In addition, Cy3labeled mRNA is readily visible under ultraviolet light without further staining after gel electrophoresis.Probes generated by these methods are suitable for multicolor fluorescence analysis, specifically dual-color expression arrays in conjunction with cyanine3-UTP .
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